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Only reads that uniquely mapped to the reference sequence chr17: 34,283,17334,286,052 of the Homo sapiens genome (Gencode, release 38) were considered for the methylation analysis
Generally, peptides are considered safe when used correctly
Qualifying conditions include type 2 diabetes, prediabetes, high blood pressure, high cholesterol, sleep apnea, PCOS, fatty liver disease, cardiovascular disease, insulin resistance, metabolic syndrome, and weight-related joint pain
One-way ANOVA showed significant differences between the groups in terms of age ( p = 0.001), hence age was included as a covariate in between group comparisons of metabolite concentrations
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